A lab experiment was conducted with the objectives of 1. Determining the efficacy of banana sap on improving physiological qualities of millets and pulse crops and 2.To standardize the optimum concentration of banana sap for different crop seeds. Selected crop seeds viz., Pearl millet (Pennisetum typoides) , kodo millet (Paspalum scrobiculatum), foxtail millet (Seteria italica), barnyard millet (Echinochloa frumentacea) pigeon pea (Cajanus cajan), mungbean (Vigna radiata), blackgram (Vigna mungo) and clusterbean (Cyamopsis tetragonoloba) were germinated in petriplates bottom lined with germination paper and watered with different concentrations of fresh banana sap (extracted by inserting a hollow metal tube into banana pseudo stem and collecting the oozed sap in plastic bottle) viz.,T1 - untreated control, T2 - sap: water ratio of 1:1 (50 %), T3 - 1:2 (33 %) and T4 - 1:3 (25 %). Seeds germinated were counted every day and at the end of germination test physiological observations viz., germination percentage, seedling length, dry matter production and vigour index were carried out. The data obtained from different parameters were analyzed by the 'F' test of significance using AGRES Statistical Package in Completely Randomized Block Design (CRD). Seeds supplied with 33% of banana sap performed significantly best in all the crop seeds except Foxtail millet (25 %), Kodo millet (25 %), pearl millet (50%) and clusterbean (50 %). It was observed that all the three concentrations of banana sap tried, improved the seed physiological quality compared to untreated control. Banana sap being a rich nutrition source of N, P, Kandanti-oxidant and anti-microbial properties might have improved the physiological qualities of seeds. It is an eco-friendly, organic seed treatment and can be recommended as pre-sowing seed treatment after standardizing soaking duration for various crops and can easily be adapted by farmers.