For the estimation of mid-parent and better-parent heterosis in Linum usitatissimum L. genotypes an experiment was conducted at Department of Agricultural Botany, College of Agriculture, Nagpur during 2009-10 and 2010-11 using line x tester analysis. All the 60 F1 hybrids and their parents (20 males + 3 females) were planted in a randomized complete block design with two replications. Out of 60 hybrids, significant negative mid-parent and better-parent heterosis were estimated in 9 and 21 hybrids for plant height, in 20 and 35 hybrids for days to 50% flowering, in 18 and 30 crosses for days to physiological maturity and in 10 and 22 crosses for budfly (Dasyneura lini) infestation, in 17 and 32 crosses for alternaria blight (Alternaria lini) respectively. Positive significant heterosis were estimated in 51 and 41 crosses for number of branches plant-1. However, significant positive mid -parent and better-parent heterosis were recorded in 57 and 55 hybrids for no of capsule plant-1, in 39 and 20 hybrids for 1000 seed weight, in 45 and 34 hybrids for yield plant-1 respectively. Among parents, Karthika, ACC NO 4/47, PKVNL-260, EC-9825 and GS-234 proved to be superior when used as parents in most of the hybrid combinations. Maximum positive significant heterosis over better-parent were observed in crosses Padmini x ACCNO4/47, Karthika x JRF-5, Padmini x LCK-8605, PKVNL-260 x ACCNO4/47, PKVNL-260 x Eita and Karthika x EC-9825 for early flowering and plant height, branches plant-1, days to physiological maturity, yield plant-1, number of capsules plant-1 and 1000 seed weight respectively. From present experiment crosses PKVNL-260 x ES-44 and PKVL-260 x EC-9825 were found best for budfly infestation and alternaria infestation respectively.