The field trial of twenty two accessions of horsegram [Macrotyloma uniflorum (Verdcourt)] was evaluated at vegetative stages to ascertain the extent of genetic diversity and access heterogencity among the Germplasm. It was concluded that high degree of allelic variation was present in seed colour, seed shape, pod shape, crop duration, primary branches, pods per plants, yields per plants, shattering and non shattering ability. The correlation among plant height, number of primary branches, number of grain per pod, 1000 grain weight, yield per plant as well as direct and indirect effects of those traits on the grains yield were investigated. These characters had significant effect on grain yield. Accessions with best performance were assorted for the desired parameters for its utilization in breeding programme.