SRAP analysis was applied in studying the diversity and genetic relationship of 20 genotypes of gladiolus. Ten morphological traits and Sequence Related Amplified Polymorphism (SRAP) analysis was carried out using twenty five primer combinations out of which, thirteen primer combinations revealed a total of 220 scorable bands, among which 176 were polymorphic with an average of 13.54 polymorphic bands per pair of primers. The percentage of polymorphism ranged from a maximum of 93.33 per cent in Me 3 + Em 3 primer combination to a minimum of 50.00 per cent by Me 2 + Em 6. For morphological data, an unweighed pair-group method using an arithmetic average was used to construct a dendrogram. The genotypes with superior commercial characters, such as spike length, rachis length, floret diameter etc. were clustered together. The SRAP profiles generated were evaluated for studying the Jaccard's similarity coefficient. The similarity matrix coefficient ranged from 18 to 79 percent, suggesting a higher genetic variation within gladiolus genotypes. On comparing the genetic diversity as revealed by the dendrogram, it was evident that Shobha a mutant genotype was identified quite distinct from other genotypes.