Research was carried out to assess the grain quality of F7 Recombinant inbred lines (RIL's) of aerobic rice compared to two parents BPT 5204 (Fine grain) and HPR 14 (High protein rice) along with two checks MAS 26 and MAS 946-1 developed at Department of Genetics and Plant Breeding, UAS, Bengaluru. Physico-chemical parameters measured were Alkali spreading test, Gelatinization temperature and amylose content. The results of the study showed significant differences (p<0.05) in case of amylose content. 58 genotypes had good amylose content ranging intermediate (20-25%),57 genotypes had shown very high amylose content (>25%) and 1 genotype was showing low amylose content (<20%). The parent varieties however, had lower levels of amylose content <20% and even checks had shown low amylose content. The parents and checks recorded higher gelatinization temperature of 75-79 0C which was good,where kernels are not effected at high gelatinization temperature. Genotypes showing medium and low gelatinization temperature exhibited kernel dispersed and intermingled means totally not fit for cooking quality. So this needs requisite attention in future breeding programmes.