Vol. 6 (3) : May-June 2015 issue
Green Farming Vol. 6 (3) : 505-508 ; May-June, 2015
Genetic diversity analysis of soybean (Glycine max L.) cultivars revealed by PCR based molecular markers
H.V. KALPANDE1*, A.W. MORE2, J.D. DESHMUKH3 and A.N. NAIKNAWARE4
Deptt. of Agricul. Botany, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani - 431 402 (Maharashtra)
Designation : 1Associate Professor *(anilgpb2011@gmail.com), 2,3Asstt. Professor, 4P.G. Student
Subject : Crop Genetics and Plant Breeding
Paper No. : P-1748
Total Pages : 4
Received : 29 March 2014
Revised accepted : 23 March 2015
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Citation :
H.V. KALPANDE, A.W. MORE, J.D. DESHMUKH and A.N. NAIKNAWARE. 2015. Genetic diversity analysis of soybean (Glycine max L.) cultivars revealed by PCR based molecular markers. Green Farming Vol. 6 (3) : 505-508 ; May-June, 2015
ABSTRACT
Genetic diversity of 10 soybean cultivars of Glycine max L. was carried out using fifty RAPD primers of OPA, OPAC, OPC, OPB, OPG, OPL, OPH, OPR, OPX series. Twelve selected polymorphic RAPD marker produced 90 fragments of which 64 fragments were found to be polymorphic, resulted in 72.74 percent polymorphism. All cultivars could be distinguished based on RAPD profiles Unweighted pair groupmethod with the airthmetric average (UPGMA) was used to construct dendrogram. The value of similarity coefficient of Dendrogram calculated by RAPD marker ranged from 0.313 to 0.618. In addition cluster analysis (CA) was used in order to determine genetic variation among soybean cultivars. The results obtained in this study showed narrow genetic base among the cultivars and can be used in selecting divergent parents for breeding and mapping purposes.
Key words :
Cluster analysis, Genetic diversity, RAPD, Soybean genotypes.
