In the present study aim was to evaluate the genetic diversity of the groundnut genotypes using Random Amplified Polymorphic DNA (RAPD) molecular marker and improve such techniques as suitable strategies for groundnut genotypes characterization. Twenty four genotypes of groundnut were included and subjected to RAPD molecular markers analysis. Out of 23 primers, 19 were amplified except P-20, OPF-17, OPK-06 and OPK-07. A total of 97 amplified bands were obtained out of which 70 were polymorphic that showed 72.16% polymorphism. Based on RAPD, the values of similarity coefficient ranged from 0.63 to 0.94 i.e., 63-94% or genetic diversity ranged from 6 to 36%. The RAPD cluster tree analysis of 24 A. hypogaea L. genotypes showed that they could be mainly divided into 4 major clusters at a similarity coefficient of 0.78. Based on dendrogram generated using UPGMA method and PCA, most of the genotypes could be placed into four main clusters. Cluster I consisted of two 2 genotypes, Cluster II had two genotypes; while cluster III included a maximum of 15 genotypes and cluster IV had only 3 genotypes. The genotype UG182 lay far apart and thus showed maximum genetic distance.