Vol. 8 (4) : July-August 2017 issue
Green Farming Vol. 8 (4) : 808-813 ; July-August, 2017
Genetic diversity of groundnut (Arachis hypogaea L.) genotypes revealed by RAPD markers
DHWANI SHARMA1*, ARUNABH JOSHI2, DEVENDRA JAIN3 and P.B. SINGH4
Department of Molecular Biology and Biotechnology, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture & Technology, Udaipur - 313 001 (Rajasthan)
1Ph.D. Scholar *(firstname.lastname@example.org), 2Professor, 3,4Asstt. Professor
Subject : Biotechnology, Molecular biology, Agriculture Microbiology and Cancer Res.
Paper No. : P-6664
Total Pages : 6
Received : 17 April 2017
Revised accepted : 30 May 2017
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DHWANI SHARMA, ARUNABH JOSHI, DEVENDRA JAIN and P.B. SINGH. 2017. Genetic diversity of groundnut (Arachis hypogaea L.) genotypes revealed by RAPD markers. Green Farming Vol. 8 (4) : 808-813 ; July-August, 2017
In the present study aim was to evaluate the genetic diversity of the groundnut genotypes using Random Amplified Polymorphic DNA (RAPD) molecular marker and improve such techniques as suitable strategies for groundnut genotypes characterization. Twenty four genotypes of groundnut were included and subjected to RAPD molecular markers analysis. Out of 23 primers, 19 were amplified except P-20, OPF-17, OPK-06 and OPK-07. A total of 97 amplified bands were obtained out of which 70 were polymorphic that showed 72.16% polymorphism. Based on RAPD, the values of similarity coefficient ranged from 0.63 to 0.94 i.e., 63-94% or genetic diversity ranged from 6 to 36%. The RAPD cluster tree analysis of 24 A. hypogaea L. genotypes showed that they could be mainly divided into 4 major clusters at a similarity coefficient of 0.78. Based on dendrogram generated using UPGMA method and PCA, most of the genotypes could be placed into four main clusters. Cluster I consisted of two 2 genotypes, Cluster II had two genotypes; while cluster III included a maximum of 15 genotypes and cluster IV had only 3 genotypes. The genotype UG182 lay far apart and thus showed maximum genetic distance.
Key words :
Arachis hypogaea, Dendrogram, PIC, RAPD, Similarity coefficient, UPGMA.