Vol. 6 (3) : May-June 2015 issue
Green Farming Vol. 6 (3) : 496-499 ; May-June, 2015
Identification and validation of informative SSR markers for genetic purity testing in hybrid rice (KRH-4)
C. PUSHPAa1*, RAME GOWDAa2, N. NETHRAa3 and N. SHIVAKUMARb4
aAICRP on Seed Technology, NSP, GKVK, University of Agricultural Sciences, Bangalore - 560 065 (Karnataka)
bZonal Agricultural Research Station, Mandya - 571 405 (Karnataka)
Designation : 1P.G. Student *(pushpa2718@gmail.com), 2,4Professor, 3Asstt. Professor
Subject : Seed Science Technology
Paper No. : P-2365
Total Pages : 4
Received : 25 July 2014
Revised accepted : 18 March 2015
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Citation :
C. PUSHPA, RAME GOWDA, N. NETHRA and N. SHIVAKUMAR. 2015. Identification and validation of informative SSR markers for genetic purity testing in hybrid rice (KRH-4). Green Farming Vol. 6 (3) : 496-499 ; May-June, 2015
ABSTRACT
Identification of parental lines/hybrid and determination of genetic purity represents an important objective for seed certification and for the control of seed in trade, assure the quality to farmers. With the objective of identifying informative SSR markers for rice hybrid KRH-4 a set of 54 SSR primers were used. Fifteen SSR primers RM206, RM276, RM202, RM204, RM263, RM216, RM219, RM6844, RM1385, RM228, RM6696, RM21 RM209, RM7279, and RM336 were polymorphic. RM21 and RM1385 can be used as unique markers when compared to other public bred hybrids to determine the genetic purity of seed lots of KRH-4. Hence, it is proposed that SSR markers can be used as an effective tool in hybrid seed purity analysis since this technique is simple to use, more accurate and not affected by environment when compared with conventional Grow out Test.
Key words :
DNA isolation, Genetic purity, Grow out test, Hybrid rice, Polymorphic markers, SSR markers.
