A field study was conducted during rabi 2008-09 at Main Research Station College of Agriculture, University of Agricultural Sciences, Dharwad to study the influence of hydrophilic polymer on biochemical and quality parameters in tomato. The treatments consisted of different concentrations of HP (Liquasorb, 0.50 to 1.75 g/plant) and another was control. These treatments were imposed at the time of transplanting of seedlings. The results of the investigation revealed that among the treatments the application of HP (1.75 g/plant) into the soil increased significantly the biochemical parameters such as total chlorophyll content (2.315mg/g fr.wt), nitrate reductase activity (118.0 n mol NO2 / g fr.wt/hr.),as well as quality parameters viz., ascorbic acid content (38.80 mg/100g fr.wt), lycopene content (4.48 mg 100 fr. wt.-1), total soluble solid(TSS) content(4.60 %), pH (4.10) and juice percent (66.01%) .The biochemical and quality parameters were significantly maximum with the application of HP (Liquasorb) (1.75 g/plant) as compared to all other treatments. The results of this study have shown quality could be improved by adding hydrophilic polymer to the soil as the polymer in soil can store extra water and enable to the plants to utilize that water over an extended period of time, which maintained the biosynthesis of enzyme in plant system .