A long-term experiment in rice–rice cropping system with twelve treatments viz., chemical fertilizers alone and in conjunction with FYM, paddy straw and gliricidia at 50% and 25% N substitution during kharif and with 100% and 50% RDF in rabi was established at the College Farm of PJTSAU, Hyderabad, during kharif 1988-89. After 25 years of experimentation, the changes in the bulk density were significant only in the upper 30 cm soil layers and ranged from 1.31 to 1.48 Mg m-3 in the surface layer (0-15 cm), from 1.58 to 1.82 Mg m-3 in the subsequent 15-30 cm layer and in lower layers (30-60 cm) the differences in BD were insignificant. A significant improvement in organic carbon was evidenced by the integrated nutrient supply through 50 or 25 per cent substitution of N fertilizer in the kharif season with FYM or paddy straw. Application of recommended dose of NPK through fertilizers significantly increased the availability of nutrients compared to control. The trend of available nitrogen over years imply that the fertility of the soil can be better sustained by substituting 25 or 50% N fertilizer with FYM or Glyricidia twigs in the kharif season than only through chemical fertilizers. Considerable build up in the available phosphorus ranging from 37.1 to 43.2 kg ha-1 was recorded with all the INM practices and was at par with 100% NPK. On an average the potassium status at the end of 25 years of rice-rice sequence was in the range of 156-195 kg ha-1and irrespective of the treatments there was depletion in the available potassium compared to initial status. Available copper and iron contents depleted in all the treatments when compared to the initial status. Manganese depletion was also seen with all other treatments except with 50% NPK+50% N substituted treatment, where in a slight build up was noticed. All the substituted treatments could maintain the zinc to its initial status and 50% NPK + 50% N substituted treatment showed a buildup. Highest dehydrogenase activity was recorded under 25% N substitution through Gliricidia while acid and alkaline phophatases activity was higher under 50% N substitution through FYM.