The present investigation was conducted to develop of in vitro salt tolerant plantlets of Jatropha curcas L. For this nodal segment from 3-5 months old nursery grown plants were used as explants for axillary bud culture. The sterilization treatment involving dipping explants in 0.1 per cent HgCl2 solution for 5 minutes resulted in minimum contamination and maximum establishment of nodal explants. The treatment MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAA was the best for culture establishment and shoot proliferation from the axillary buds. Shoots at 0.25 per cent, 0.5 per cent and 0.75 per cent survived when the established shoots were treated with different concentration of NaCl starting from 0.25 per cent to 2.0 per cent. The salt tolerant shoots produced roots when treated with half MS media fortified with 1 mg/L IBA, 3 mg/L NAA and 0.25 g AC up to 0.5 per cent where as under 0.75 per cent NaCl rooting failed completely. The rooting per cent and root length were less under 0.25 per cent and 0.50 per cent NaCl. It shows MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAA and half MS media fortified with 1 mg/L IBA, 3 mg/L NAA and 0.25 g AC are best in shoot establishment and root development respectively for developing salt tolerant plantlets in vitro.