Vol. 7 (2) : March-April 2016 issue
Green Farming Vol. 7 (2) : 498-500 ; March-April, 2016
In vitro propagation studies in pomegranate (Punica granatum L.) cv. ‘Bhagwa’
AKSHATA HOSAMANI1*, MANJULA V.2, GEETHA, M.S.3, PRAKASH KERURE4 and SUKHADA MOHANDAS5
Division of Plant Biotechnology, Indian Instt. of Horticultural Res., Hesaraghatta, Bengaluru - 560 089 (Karnataka)
Designation : 1Asstt. Agriculture Officer, 2,3SMS, 5Principal Scientist, 4Asstt. Professor *(prakash837@gmail.com)
Subject : Biotechnology, Molecular biology, Agriculture Microbiology and Cancer Res.
Paper No. : P-3793
Total Pages : 3
Received : 15 June 2015
Revised accepted : 27 February 2016
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Citation :
AKSHATA HOSAMANI, MANJULA V., GEETHA, M.S., PRAKASH KERURE and SUKHADA MOHANDAS. 2016. In vitro propagation studies in pomegranate (Punica granatum L.) cv. ‘Bhagwa’. Green Farming Vol. 7 (2) : 498-500 ; March-April, 2016
ABSTRACT
Micro propagation in fruit tree would help in overcoming difficulties of vegetative propagation, producing true to type plants and rapid mass production of planting materials. The present study revealed that, the reliable and reproducible protocols were developed to get healthy and well formed plants from nodal and leaf explants of the pomegranate(Punica granatum L.) cv Bhagwa. Completely sterilized nodes were inoculated on MS media supplemented with BAP ranging from 0.3-2.1mg l-1 and NAA (0.2 mg l-1 and 0.4 mg l-1) and leaves with BAP ranging from 0.5-2.5 mg l-1 and NAA (1mg l-1 and 2 mg l-1). Callus obtained from both the explants were subculture on the same medium for callus proliferation and shoot regeneration. Regenerated shoots were rooted on 1/2MS medium supplemented with different concentrations of NAA and IBA ranging from 0.25?M to 2.0?M and activated charcoal 50 mg l-1. Highest growth response from nodal explant was obtained in MS medium containing 1.8 mg l-1 BAP and 0.4 mg l-1 NAA with the regeneration efficiency of 90%. MS medium supplemented with 2 mg l-1 BAP and 2 mg l-1 NAA stimulated shoot regeneration in leaf. Rooting was best observed in IBA 0.5mgl-1 from shoots derived from axillary buds of nodal explants, whereas 0.25mg of NAA was found to be best for leaf and derived plantlets.
Key words :
BAP, Explants, Growth regulators, In-vitro, NAA, Propagation, Pomegranate.