In present study, an experiment was carried out in complete randomized design (CRD) with 3 replications, where unpollinated ovaries were excised from four varieties of wheat (Triticum spp.) two varieties from each of bread wheat and durum; GW 366, GW 322, HI 8498 and HI 8381 respectively. It was observed that genotypes, stages of harvesting, types of media, combinations of PGRs and durations of cold pretreatment at 4oC significantly affected formation of embryonic tissues independently. Stage II was taken as the best stage for induction of embryonic tissues. For induction of embryonic tissues, light culture conditions, 30 g/l of maltose, MS medium, and 15 days of cold pretreatment were the best conditions. From 12 combinations of 2,4-D and KIN supplemented in MS basal medium, 1 mg/l of each of 2,4-D and KIN was found to be the best combination for induction of embryonic tissues for varieties HI 8381 (42.0 %), GW 366 (34.8%) and HI 8498 (16.9 %). Ten different PGR combinations were used for shoot regeneration, 0.1 mg/l of NAA + 1 mg/l KIN was the PGR combination where the embryonic tissues of all varieties regenerated into shoots. The highest frequency of shoots were regenerated from the cultured embryonic tissues of varieties GW 366 (54.5 %) and HI 8381 (43.26 %) at 0.1 mg/l 2,4-D. From a total of 15,921 cultured ovaries, 1303 embryonic tissues and 207 regenerants were obtained. The average percentage of embryonic tissues and regenerants were 9.3 % and 2.9 % from 4,136; 10.2 % and 0.87 % from 5,157; 6.7 % and 0.24 % from 3,161; 5.2 % and 0.99 % from 3,467 cultured ovaries for varieties HI 8381, GW 366, HI 8498 and GW 322 respectively. All plantlets transferred into pots were survived. Except three plantlets, remaining survived in the glasshouse and grown till maturity. Further, crossing attempt between durum and bread wheat may be improve the quality of wheat.