Jamun (Syzygium cuminii L.) efficiently propagated in vitro through sigle nodal segment and was described by axillary bud proliferation. Multiple shoots were induced from single nodal segments when cultured on full strength MS (Murashige and Skoog) medium. Single nodal segments from seedlings established better in vertical position and shown good establishement, on surface sterilization with mercuric chloride (0.5 %, 11 min) and ethanol at (70 %, 30 sec) and cultured on full strength MS medium along with 3 % sucrose, 2mg/l BAP, 100 mg/l ascorbic acid produced an average of 32 shoots per explants after 6th subculture at 12th week. The shoots were rooted in 1/4th strength MS media with 3% activated charcoal , IBA (25 mg/l) induced early rooting within 13 days, with a single root and later with a higher root length of (5.7 cm) after 7th week of the transfer and maximum number of roots (5.5) were obtained in 2.5 mg/l IBA. The rooted one were hardened and vermiculite was proved to be better with (35.5 %) of survival after 6th week. Then transferred to the polybags and planted in the field.