One of main approaches are being used to improve salt tolerance is the generation of transgenic plants to introduce novel genes or to alter expression levels of the existing genes to increase the degree of salt stress tolerance. Because salinity stress affects the cellular gene-expression machinery, it is evident that molecules involved in nucleic acid processing, including helicases, are likely to be affected as well. Overexpresion of the PDH45 (Pea DNA helicase 45) in tobacco showed increased tolerant to salinity stress. Hence in this direction we made an attempt to develop transgenics in finger millet (Eleusine coracana (L.) Gaertn) over expressing PDH45 and for this Agrobacterium mediated gene transfer method/in vitro was adopted. Finger millet transgenics were successfully developed by invitro method method using seed as explant in callus regeneration method. Successful transformation at callus stage was initially confirmed by GUS assay. And by PCR amplification genomic DNA of putative transformed calli showed positive for hptII and gene specific primers. And the results by RT-PCR showed that the level of transcripts overexpression in transformed calli was relatively higher than 150mM and 200mM salt stressed non-transformants control calli. Finally putative transgenics were confirmed by PCR amplifying the genomic DNA of putative transformed T0 plants.