An investigation was conducted to determine the influence of nitrogen nutrition on shelf life of cucurbitaceous vegetable, bittergourd (Momordica charantia L.) using farmyard manure as an organic source. Three levels of nitrogen (200, 250 and 300 kg Nha-1) were applied in three ratios of organic-chemical N substitutions (1:1, 1:2, 2:1) at three different frequencies of application on equivalent N basis. The experiment was laid out as confounded 33design in 9-plot blocks with 2 replications confounding LR2F in RI and LRF2 in RII. Fruits could be stored for more days at ambient temperature when nutrient N was supplied through 2:1 ratio of farmyard manure and chemical fertilizer. Storage life of fruits at ambient conditions was more with frequent split application of nutrient N. Shelf life of the fruits was favourably affected when low levels of nutrient N was applied through 2:1 ratio of organic and chemical fertilizer N.