The field performance of pollen grains was observed by the application of pollens stored at different proportion of filler mixture (1 g borax + 99 g rice flour) on female parent VN-2 during hybrid seed production of HCH-9646. In vivo pollen viability studies revealed that there was significant difference in filler mixture for number of crossed fruits retained per plant and fruit set (%) and the values were higher in cent per cent pollen. Irrespective of filler mixture proportion fresh pollens recorded higher number of crossed fruits retained per plant (23.16) and fruit set (33.06%) followed by pollens stored in refrigerator for one day over other storage methods. Laboratory experiment was conducted to know the viability of pollen grains of Arka lohit. In vitro studies on pollen viability indicated that pollens were very susceptible to storage under ambient conditions when compared to pollen stored in refrigerator or moist earthen pot. The interaction revealed that 75 per cent pollen and 25 per cent filler mixture stored in refrigerator was equally better to fresh pollen combinations and there was a very slight reduction in pollen germinability over a period of one day, this was followed by pollen stored in earthen pot.