Present experiment was conducted on the Student's Instructional Farm during 2006-07 for the quality improvement of wheat grain in N.D. University of Agriculture & Technology, Kumarganj, Faizabad. All the treatments were recorded for significantly improvement the grain quality of wheat by the application of sulphur. Application of sulphur @ 20, 40, 60 kg/ha improved the grain quality than control. Sulphur is a major factor for the improvement of wheat seed quality and wheat flour product. Sulphur significantly influenced the protein content dry & wet gluten content, total, reducing and non reducing sugar content, amino acid content and also total mineral content. Sulphur being the structural constituent of some amino acid, protein, enzyme and co-factor, which influence the sugar synthesis metabolism protein synthesis, water absorption capacity and also their mineral content.