The present investigation “Influence of various growing medias and size of polybags on the growth, buddability and budding success in Rough lemon” was carried out at new Nursery, Department of Horticulture, Khalsa College, Amritsar during 2015-2016. The experiment was laid out in RBD factorial design with eighteen treatment combinations comprised of six growing medias as M1 (Soil : cocopeat : FYM :: 1: 1: 1), M2 (Soil : vermicompost : cocopeat :: 1: 1: 1), M3 (Soil : FYM :: 1: 1), M4 (Soil : vermicompost :: 1: 1), M5 (Soil : cocopeat :: 1: 1), M6 (Soil) and three size of polybags viz. 8' × 8' , 10' × 10' and 12' × 12'. Twenty seedlings were considered as one replication. The growth of seedlings improved significantly when raised in 12' × 12' sized polybags having growing media of Soil + Cocopeat + FYM as compared to other treatment combinations. Maximum seedling height (85.10 cm), number of shoots (3.40), stem thickness (1.00 cm), number of leaves (92.60), root length (17.64 cm), number of primary roots (50.80), number of secondary roots (315.20), fresh (8.62 g) and dry weight of roots (7.30 g) was found in the seedlings raised in 12' × 12' polybags with soil + Cocopeat + FYM. Moreover, the seedlings raised in polybags of size 12' × 12' with growing media M1 achieved buddable size early than other seedlings grown in rest of the medias. These seedlings had also maximum bud intake percentage and took minimum days for bud sprouting than the other seedlings raised in respective growing medias.