Vol. 6 (3) : May-June 2015 issue
Green Farming Vol. 6 (3) : 587-589 ; May-June, 2015
Isolation and characterization of Gluconacetobacter sp. from fruit sources
SUDHEERKUMAR TALAWAR1*, B. NARAYANASWAMY2 and S.N. RAVINDRA3
Deptt. of Agricultural Microbiology, University of Agricultural Sciences, GKVK, Bengaluru - 560 065 (Karnataka)
Designation : 1P.G. Student *(sudeer.micro@gmail.com), 2Associate Professor, 3Research Scholar
Subject : Biotechnology, Molecular biology, Agriculture Microbiology and Cancer Res.
Paper No. : P-3152
Total Pages : 3
Received : 09 January 2015
Revised accepted : 30 April 2015
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Citation :
SUDHEERKUMAR TALAWAR, B. NARAYANASWAMY and S.N. RAVINDRA. 2015. Isolation and characterization of Gluconacetobacter sp. from fruit sources. Green Farming Vol. 6 (3) : 587-589 ; May-June, 2015
ABSTRACT
Gluconacetobacter sp. were isolated from rotting sugary fruits. Four hundred and thirty two samples consisting of rotting sugary fruits were tested for isolation among them 32 apple fruits showed good results for isolation of Gluconacetobacter sp. The Gluconacetobacter sp. grown in Tender coconut milk broth (TCMB), containing 9% refined sugar, 0.5% ammonium dihydrogen orthophosphate and 2% glacial acetic acid was added. The isolates showed positive growth at 30° C and also at pH 4.5 and showed negative growth at 35oC and at pH 5.5. The isolates were found to be short rods, occur either singly or in pairs, and showed negative gram reaction. The isolates showed positive for acid production and negative for gas production, indole production, nitrate reduction, gelatin hydrolysis. The isolates obtained showed positive for catalase test. All these morphological and biochemical examination indicated that the isolates belongs to the genus Gluconacetobacter.
Key words :
Enrichment culture technique, Gluconacetobacter, Tender coconut milk broth (TCMB).
