Vol. 6 (3) : May-June 2015 issue
Green Farming Vol. 6 (3) : 583-586 ; May-June, 2015
Isolation & characterization of efficient exopolysaccharide producing bacteria from rhizosphere of wheat, maize and mungbean crop grown in rainfed condition
DEVENDRA SINGH1*, GEETA SINGH2 and K. ANNAPURNA3
Division of Microbiology, Indian Agricultural Research Institute, Pusa, New Delhi - 110 012 (India)
Designation : 1Ph.D. Scholar *(devendraiari@gmail.com), 2Principal Scientist, 3Scientist
Subject : Biotechnology, Molecular biology, Agriculture Microbiology and Cancer Res.
Paper No. : P-2304
Total Pages : 4
Received : 14 July 2014
Revised accepted : 02 March 2015
Get Access
Citation :
DEVENDRA SINGH, GEETA SINGH and K. ANNAPURNA. 2015. Isolation & characterization of efficient exopolysaccharide producing bacteria from rhizosphere of wheat, maize and mungbean crop grown in rainfed condition. Green Farming Vol. 6 (3) : 583-586 ; May-June, 2015
ABSTRACT
Under laboratory condition exopolysaccharides (EPS) producing bacteria were isolated from the rhizosphere soil samples of wheat, maize and mungbean grown under rainfed condition and were screened in vitro for moisture stress tolerance in nutrient broth supplemented with different concentrations of polyethylene glycol (PEG 6000) during (2012-2013) at IARI, New Delhi, India. In present study, total 26 isolates (9 isolate from wheat, 7 isolates from maize and 10 isolates from mungbean rhizosphere, were found to tolerate maximum level of stress (45% PEG 6000) in broth culture . These isolates were monitored for the amount of EPS produced under maximum level of polyethylene glycol. The isolate G7, M5, and MU9 were found to be high EPS producing isolates among the wheat, maize and mungbean isolates respectively. These isolates were identified as Pseudomonas sp, Bacillus cereus and Microbacterium resistens respectively on the basis of morphological, biochemical, and 16S rDNA sequence analysis.
Key words :
16S rDNA, Exopolysaccharide, Moisture stress, PEG, Rhizosphere.
