The following study illustrates a successful protocol for micropropagation of wilt resistant hybrid rootstock of guava using leaf-induced calli. Explant survival of 43.30 per cent and 21.03 per cent callusing frequency was obtained in the duration from March to May and contamination levels were as low as 23.67 per cent. Pre-treatment of the explants with a combination of Carbendazim 0.1%+0.05% Metalaxyl and surface sterilization with 0.1% HgCl2 was most effective in controlling contamination and giving high survival rate (40.10%). 1.5 cm2 was found to be optimum size for callus induction (21.27%) and low necrosis (21.67%). Out of the different combinations of auxin and cytokinin tried, 2ip 0.5mg/l + NAA 0.5mg/l containing media gave 63.43 per cent survival of explants, high regeneration frequency (32.12%) and required only 12.7 days for callusing. Murashige and Skoog basal media was found most suitable, giving rise to maximum number of calli from explant (48.20%) in 12.7 days and 10.70 shoots per callus. Even though in vitro leaves were better in terms of number of days taken for callusing and for conversion, the calli from ex vitro leaves were better and shoots regenerated from them were more stable.