Seven media were tested for mass multiplication of Pseudomonas aeruginosa, among the natural media, coconut water (143.66) induce maximum colony count. In sterile condition, among the different concentrations of coconut water tested, 30 per cent concentration showed significantly more population of P. aeruginosa (389). In unsterile condition among the different concentrations of coconut water tested, 30 per cent concentration showed significantly higher population of P. aeruginosa (455.33) as compared to rest. The shelf-life study done in conical flask and HDPE bottles, in conical flask at fifteen days of storage of coconut water formulation, the population of P. aeruginosa at 10-7 dilution was recorded as 94.00. It was found to increase upto 30 days and reached at it's highest (128.00). Then after it was observed to decrease upto 180 days (2.00). The population recorded at 180 days of storage (2.00) at room temperature (25±2oC). While in HDPE bottles, at fifteen days of storage of coconut water formulation the population of P. aeruginosa at 10-7 dilution was reached at 99.00 then increasing upto 30 days and reached at it's highest (125.00), then after it was observed to decrease upto180 days (1.00). The population recorded (1.00) at 180 days of storage at room temperature (25±2oC). The highest cfu/ml was recorded at 30 days of storage (1.25 x 1010 cfu/ml ) while the lowest (1 x 108 cfu/ml) at 180 days of storage.