Vol. 8 (1) : January-February 2017 issue
Green Farming Vol. 8 (1) : 214-217 ; January-February, 2017
Mass multiplication and shelf-life study of liquid formulation of Pseudomonas sp. & their effect on PGPR activity and blast disease of finger millet
MANISHA S. SHINDEa1*, A.N. SABALPARAa2 and A.J. DESHMUKHb3
aDepartment of Plant Pathology, N. M. College of Agriculture, Navsari Agricultural University, Navsari - 396 450
bAgriculture Polytechnic, Waghai, Navsari Agricultural University, Navsari - 396 450 (Gujarat)
Designation : 1Ph.D. Scholar *(manisha5476@gmail.com), 2Director of Research & Dean, 3Asstt. Professor
Subject : Plant Pathology, Nematology and Virology, Mushroom Cultivation
Paper No. : P-5154
Total Pages : 4
Received : 11 May 2016
Revised accepted : 16 December 2016
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Citation :
MANISHA S. SHINDE, A.N. SABALPARA and A.J. DESHMUKH. 2017. Mass multiplication and shelf-life study of liquid formulation of Pseudomonas sp. & their effect on PGPR activity and blast disease of finger millet. Green Farming Vol. 8 (1) : 214-217 ; January-February, 2017
ABSTRACT
Studies were carried out to evaluate mass multiplication, shelf-life and Plant growth promoting characteristic with the formulation of Pseudomonas sp. stored at 25±20C over a storage period of seven days in a natural source (Coconut water). Coconut fruit a natural formulation was made and found that the population of Pseudomonas sp. was increased significantly, maximum population of Pseudomonas sp. was recorded when 3 ml suspension of Pseudomonas sp. was injected into intact coconut fruit (2.98 x 1010 cfu/ml). In shelf-life study, the population of Pseudomonas sp. was increase on first day (2.22 x 1010 cfu/ml) of inoculation and reached it's highest. Then after, it was observed to decrease upto 7th days (2.2 x 108 cfu/ml). Thus the prepared natural formulation can be stored for 7 days. The proposed protocol of coconut water formulation suggested is absolutely cost effective and easy to prepared at small scale level. In PGPR activity of finger millet, maximum germination (93.67 %), root (8.60 cm) and shoot (5.50 cm) length and root bacterization (5.33 x 104 cfu/g) was recorded in intact coconut water formulation Pseudomonas sp. There was significantly lower leaf blast intensity was recorded in coconut water intact formulation of Pseudomonas sp. (6.79 PDI).
Key words :
Coconut water, Finger millet, Mass multiplication, Pseudomonas sp., PGPR and Blast, Shelf-life.