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International Journal of Applied Agricultural & Horticultural Sciences
  • 29 April, 2024
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Frequency : Bimonthly
Language : English
DOI Prefix : 10.37322
P-ISSN : 0974-0775
E-ISSN : 2582-4198
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  • 1. Papers are invited for the forthcoming issues of Green Farming. Few Mini Review articles on applied aspects of new approaches (with Sr. Authors) may be adjusted, if sent on priority by email. For more details, please contact us.
Vol. 8 (1) : January-February 2017 issue
Green Farming Vol. 8 (1) : 214-217 ; January-February, 2017
Mass multiplication and shelf-life study of liquid formulation of Pseudomonas sp. & their effect on PGPR activity and blast disease of finger millet
MANISHA S. SHINDEa1*, A.N. SABALPARAa2 and A.J. DESHMUKHb3
aDepartment of Plant Pathology, N. M. College of Agriculture, Navsari Agricultural University, Navsari - 396 450
bAgriculture Polytechnic, Waghai, Navsari Agricultural University, Navsari - 396 450 (Gujarat)
Designation :  
1Ph.D. Scholar *(manisha5476@gmail.com), 2Director of Research & Dean, 3Asstt. Professor
Subject : Plant Pathology, Nematology and Virology, Mushroom Cultivation
Paper No. : P-5154
Total Pages : 4
Received : 11 May 2016
Revised accepted : 16 December 2016
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Citation :

MANISHA S. SHINDE, A.N. SABALPARA and A.J. DESHMUKH. 2017. Mass multiplication and shelf-life study of liquid formulation of Pseudomonas sp. & their effect on PGPR activity and blast disease of finger millet. Green Farming Vol. 8 (1) : 214-217  ;  January-February, 2017

ABSTRACT
Studies were carried out to evaluate mass multiplication, shelf-life and Plant growth promoting characteristic with the formulation of Pseudomonas sp. stored at 25±20C over a storage period of seven days in a natural source (Coconut water). Coconut fruit a natural formulation was made and found that the population of Pseudomonas sp. was increased significantly, maximum population of Pseudomonas sp. was recorded when 3 ml suspension of Pseudomonas sp. was injected into intact coconut fruit (2.98 x 1010 cfu/ml). In shelf-life study, the population of Pseudomonas sp. was increase on first day (2.22 x 1010 cfu/ml) of inoculation and reached it's highest. Then after, it was observed to decrease upto 7th days (2.2 x 108 cfu/ml). Thus the prepared natural formulation can be stored for 7 days. The proposed protocol of coconut water formulation suggested is absolutely cost effective and easy to prepared at small scale level. In PGPR activity of finger millet, maximum germination (93.67 %), root (8.60 cm) and shoot (5.50 cm) length and root bacterization (5.33 x 104 cfu/g) was recorded in intact coconut water formulation Pseudomonas sp. There was significantly lower leaf blast intensity was recorded in coconut water intact formulation of Pseudomonas sp. (6.79 PDI).
Key words :
Coconut water, Finger millet, Mass multiplication, Pseudomonas sp., PGPR and Blast, Shelf-life.