Inter-specific cross of Gossypium hirsutum var Sahana and Gossypium arboreum var K 10 yielded the unstable triploid and sterile derivatives. The ploidy level of this triploid (3x) was then doubled with colchicine to get an unstable hexaploid derivative. The paper presents the cyto-morphological features of the produced unstable derivatives so as to frame a suitable breeding strategy to evolve the stable culture tolerant to biotic and abiotic stresses. Phenotypic attributes like leaf lobe, length of calyx tube, petal eyespot, poor pollen fertility and abortive ovarian system were found to be dominant in the triploid. Chromosome doubled triploid progeny possessed leaves with larger veins, reduced size of epi-calyx, larger petals, and shriveled anthers and reduced pollen fertility as compared to triploid and parent. The normal meiotic behaviour was witnessed among parents while abnormal meiotic system as evidenced from the formation of trivalents and quadrivalents was noticed among triploid and hexaploid. Abnormal sporads also recorded among triploid and hexaploid leading to formation of sterile pollen grain.