Vol. 2 (5) : September-October 2011 issue
Green Farming Vol. 2 (5) : 537-539 (September-October, 2011) (New Series)
Micropropagation studies in sarpagandha (Rauvolfia serpentina L.)
S.U. KOSKEWARa1, D.S. PATILb2*, N.S. PARDEa3, S.J. THOKALEc4 and S.V. PAWARc5
aDeptt. of Biotechnology, bDeptt. of Horticulture, cDepartment of Botany, M.P. Krishi Vidyapeeth, Rahuri - 413 722 (M.S.)
Designation : 1,4Student (Agril. Biotechnology), 3Ph.D. Scholar (Agric. Botany), 2Ph.D. Scholar (Hort.) *(dinar_2007@rediffmail.com), 5Asstt. Prof. (Botany)
Subject : Biotechnology, Molecular biology, Agriculture Microbiology and Cancer Res.
Paper No. :
Total Pages : 3
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Revised accepted :
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Citation :
S.U. KOSKEWAR, D.S. PATIL, N.S. PARDE, S.J. THOKALE and S.V. PAWAR. 2011. Micropropagation studies in sarpagandha (Rauvolfia serpentina L.). Green Farming Vol. 2 (5) : 537-539 (September-October, 2011) (New Series)
ABSTRACT
The present investigation on micropropagation studies in sarpagandha (Rauvolfia serpentina L.) was undertaken to identify suitable explant and media for micropropagation. The explants of R. serpentina were collected from Medicinal and Aromatic Plat Project, MPKV, Rahuri, Dist. Ahmednagar. Among the different media concentration tried for establishment of culture, the media MS + BAP 2.0 mg/l gave maximum percent establishment i.e. 60 % for shoot tip and 50 % for nodal segment. MS + BAP 2.0 mg/I proved superior for establishment and required least days for establishment i.e. 15 days for shoot tip and 18 days for nodal segment. The media MS + BAP 2.0 mg/l + NAA 0.3 mg/l gave the maximum number of multiple shoots per culture i.e. 4 and 3 to shoot tip and nodal segment respectively after establishment of explant. Same media give maximum percent of shoot multiplication, which is 62.2 % and 56.4 for shoot tip and nodal segment respectively. The optimums rooting to the shoot were recorded on ½ MS supplemented with 2.0 mg/l NAA. Out of all rooted shoots about 66.67% plantlets were established after hardening.
Key words :
In vitro, micropropagation, Rauvolfia serpentina, plant growth regulator, sarpagandha.
