Vol. 9 (2) : March-April 2018 issue
Green Farming Vol. 9 (2) : 357-360 ; March-April, 2018
Molecular characterization of Fusarium pallidoroseum isolates by using RAPD technique
T.T. BARIA1* and R.K. PATIL2
Department of Plant Pathology, B.A. College of Agriculture, Anand Agric. University, Anand - 388 110 (Gujarat)
Designation : 1Student *(bariatushar4@gmail.com), 2Retd. Professor
Subject : Plant Pathology, Nematology and Virology, Mushroom Cultivation
Paper No. : P-7156
Total Pages : 4
Received : 24 January 2018
Revised accepted : 20 March 2018
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Citation :
T.T. BARIA and R.K. PATIL. 2018. Molecular characterization of Fusarium pallidoroseum isolates by using RAPD technique. Green Farming Vol. 9 (2) : 357-360 ; March-April, 2018
ABSTRACT
DNA analysis of seven isolates with 10 primers showed amplification of total 167 bands, with a range of 16 to 17 bands per primer. Overall, Fusarium pallidoroseum isolates exhibited a moderate level of genetic diversity. The maximum 20 bands were generated by a primer OPF- 7. Clustering pattern of dendrogram generated by using the pooled molecular data of 10 RAPD loci indicated that two clusters namely 1 and 2 were formed. Cluster 1 includes five isolates viz., SRT, AND, AMD, LND and DSA, cluster 2 includes two isolates DHD and VSN. The similarity coefficient ranged from 0.19 to 0.36 with all the 10 primers. The highest polymorphism (100 %) was showed by the primer OPA-2, OPA-6, OPE-1, OPE-5, OPF-2, OPF-3 Whereas, the lowest polymorphism (93.75%) was observed in the primer OPA-3, OPA-5 andOPF-8.
Key words :
Citrus, Fusarium pallidoroseum, Fruit rot, Post-harvest, RAPD technique.
