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International Journal of Applied Agricultural & Horticultural Sciences
  • 26 April, 2024
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DOI Prefix : 10.37322
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Vol. 7 (6) : November-December 2016 issue
Green Farming Vol. 7 (6) : 1326-1330 ; November-December, 2016
Molecular marker based genetic diversity analysis in groundnut (Arachis hypogea L.)
ASHUTOSH KUSHWAH1*, SOMA GUPTA2, K. PRADHAN3 and KULDEEP KAUR4
Deptt. of Plant Breeding & Genetics, Odisha University of Agricultural & Techn., Bhubaneswar - 751 003 (Odisha)
Designation :  
1,2,4Ph.D. Scholar *(anshu.kushwah24@gmail.com), 3Associate Professor
Subject : Crop Genetics and Plant Breeding
Paper No. : P-5598
Total Pages : 5
Received : 28 July 2016
Revised accepted : 20 October 2016
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Citation :

ASHUTOSH KUSHWAH, SOMA GUPTA, K. PRADHAN and KULDEEP KAUR. 2016. Molecular marker based genetic diversity analysis in groundnut (Arachis hypogea L.). Green Farming Vol. 7 (6) : 1326-1330 ; November-December, 2016

ABSTRACT
Molecular genetic fingerprints of 14 groundnut germplasms were developed using Random Amplified Polymorphic DNA (RAPD) markers to elucidate the genetic diversity/relatedness among the species. A total of 27 bands were detected ranging in size from 200 bp to 2250 bp among the 14 genotypes and all 27 (100%) were found to be polymorphic. Genetic relationship between groundnut genotypes were determined on the basis of Jaccard's pair wise similarity coefficients. The range of pair wise similarity varies from 0.00 to 0.833. Highest similarity coefficient was observed between 'Dh-204' and 'Dh-209' (0.833), indicating that they are belonging to similar genetic background, while minimum similarity was observed in between 'ICGV-95401' and 'DhS-102'(0.067). Following data analysis based on Jaccard's similarity coefficient, a dendrogram was plotted for the data set. It depicted that, at genetic distance 0.00, two distinct clusters were formed having single root. Two major clusters (I-II) were identified from the dendrogram, which were found distinct from each other having only 0% similarity. The highest number of genotypes (13) was included in the Cluster I whereas cluster II was represented by only one genotypes. The results of Principle component analysis were very much similar to UPGMA results. Two Dimensional and Three Dimensional scaling showed four clusters (I to IV). Cluster-I had six accession, cluster-II had three accessions, cluster-III had four accessions and cluster-IV had only one accession. Based on study the large range of similarity values for related accessions using RAPD provides greater confidence for the assessment of genetic diversity and relationships.
Key words :
Dendrogram, Genetic diversity, Groundnut, Principle component analysis, RAPD.