Vol. 3 (4) : July-August 2012 issue
Green Farming Vol. 3 (4) : 384-388 (July-August, 2012)
Molecular variance dissection among F11 recombinant lines of G. hirsutum x G. barbadense cross using RAPD marker technology
V.M. CHOUDKI1*, PRASHANTH SANGANNAVAR2, S. ANUPAMA3, S.G. SAVITA4, H.M. VAMADEVAIAH5, B.M. KHADI6 and I.S. KATAGERI7+
Deptt. of Genetics and Plant Breeding, University of Agricultural Sciences, Agricul. Res. Station, Dharwad Farm - 580 007 (Karnataka)
Designation : 1SRF *(vittalcm@gmail.com), 2Ph.D. Scholar, 3,4RA, 5Bio-Chemist, 6Dean, 7Plant Scientist +(ikatageri@yahoo.com)
Subject : Crop Genetics and Plant Breeding
Paper No. :
Total Pages : 5
Received : 29 May 2012
Revised accepted : 20 July 2012
First Page
Citation :
V.M. CHOUDKI, PRASHANTH SANGANNAVAR, S. ANUPAMA, S.G. SAVITA, H.M. VAMADEVAIAH, B.M. KHADI and I.S. KATAGERI. 2012. Molecular variance dissection among F11 recombinant lines of G. hirsutum x G. barbadense cross using RAPD marker technology. Green Farming Vol. 3 (4) : 384-388 ; July-August, 2012
ABSTRACT
Creation and assessment of genetic diversity is primary and most essential activity in crop improvement. Morphological features are indicative of the genotype but are represented by only a few loci and are highly influenced by environmental factors. Molecular markers like RAPD are plenty in number and are not influenced by environment. Their use in genetic diversity assessment is increasingly becoming popular among geneticist and breeders. Molecular variance dissection was carried out using Fourty eight F11 recombinant lines of G. hirsutum var. DS-28 x G. barbadense var. SBYF-425 cross using RAPD markers at ARS, Dharwad Farm during 2010. As many as 231 RAPD's were amplified out of which 201 (87.0%) were polymorphic with an average of 5.0 polymorphic fragments per primer. Total number of fragments amplified for each primer ranged from 4 (OPI-1, 6, 15, 19, OPE-3, OPL-13 and OPAP-7) to 10 (OPL-7) with an average of 5.8 fragments per primer. Line HBS-4 showed lowest number of amplified fragments (198), whereas highest number of amplified fragments (205) was shown by HBS-10. The similarity coefficient ranged from 0.38 to 0.93. Presence of maximum RAPD molecular variance (93%) between HBS-10 and HBS-4 and minimum molecular variance (38%) between HBS-4 and HBS-9 was evident. The dendrogram revealed two distinct clusters, one cluster formed by a single genotype HBS-10 and other cluster includes remaining 47 genotypes.
Key words :
Cotton, Gossypium hirsutum, Introgression, Molecular variance, RAPD markers.
