Vol. 3 (5) : September-October 2012 issue
Green Farming Vol. 3 (5) : 511-514 (September-October, 2012)
Molecular v/s morphological character based genetic diversity estimates in elite cotton lines
B. NAGARAJ1, S.G. SAVITA2, PRASHANTH SANGANNAVAR3, V.M. CHOUDKI4*, H.M. VAMADEVAIAH5, B.M. KHADI6 and I.S. KATAGERI7
Agricultural Research Station, Dharwad Farm, University of Agricultural Sciences, Dharwad - 580 007 (Karnataka)
Designation : 1Scientist - 'D', 2R.A., 3Ph.D. Scholar, 4SRF *(vittalcm@gmail.com), 5Bio-Chemist, 6Dean (P.G. Studies), 7Plant Scientist (ikatageri@yahoo.com)
Subject : Crop Genetics and Plant Breeding
Paper No. :
Total Pages : 4
Received : 9 July 2012
Revised accepted : 14 September 2012
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Citation :
B. NAGARAJ, S.G. SAVITA, PRASHANTH SANGANNAVAR, V.M. CHOUDKI, H.M. VAMADEVAIAH, B.M. KHADI and I.S. KATAGERI. 2018. Molecular v/s morphological character based genetic diversity estimates in elite cotton lines. Green Farming Vol. 3 (5) : 511-514 ; September-October, 2012
ABSTRACT
Information on genetic relationship among genotypes is of great importance to breeders. Genetic diversity of 20 elite cotton lines obtained from different cotton growing zones of India was investigated at the DNA level with the random amplified polymorphic DNA (RAPD) procedure and at the morphological level. Out of eighty random decamer primers screened, 22 were found to be polymorphic and they generated 146 amplicons and 93 of them were polymorphic (63.70%). Primers, OPC-08 and OPN-05 recorded highest percent polymorphism (87.50%). The genotypes CCH-3047 and Sahana showed highest taxonomic distance (87.36) but based on genetic distance analysis they are not much diverse (0.08) genotypes. Similarly, genotypes AKH-05-6 and F-2178 showed highest genetic distance (0.25) but based on taxonomic distance analysis they are not much diverse (32.61) genotypes. In another case the genotypes, CCHLS-4 and GSHU-154 recorded least taxonomic distance, but the same genotype recorded more genetic distance (0.15) and the same is shown in dendrograms. The overall correlation coefficient between taxonomic and genetic distance was -0.01, suggesting non-existence of association between these two measures of diversity.
Key words :
Correlation coefficient, Cotton, Genetic diversity, Polymerase chain reaction (PCR), Random amplified polymorphic DNA (RAPD).
