Sericulture provides self employment and highly remunerative industry and happens to be main occupation in many parts of Karnataka, India. Mulberry being perennial in nature is cultivated as seasonal crop for feeding silkworm to generate cocoon production. Evaluation of qualitatively high yielding mulberry varieties for region and season specific is priority of research. Different genotypes are available in nature with varying chromosome number makes the mulberry to behave phenotypically and genotypically different. Diploids though more suitable to silkworm because of its increases stomatal number and frequency, decreased trichome number and leaf thickness when compared to tetraploids from the palatability point of view. Triploids are more superior in producing quality cocoons compared to diploids and tetraploids. In the present investigation number of stomata in M5 was more (150.1±4.1) compared to colchiploid (48.8±1.64) where as triploid exhibited intermediate values (91.2±2.38). Stomatal frequency also varies and showed an increasing trend from diploid M5 (435.07±0.83mm2) to triploid (327.10±2.74mm2) and colchiploid (123.53±0.33mm2). Number of trichomes indicated decreased trend from diploid (8.6±0.54) to colchiploid (16.8±1.30). The thickness of leaf is more in colchiploids (306.8±0.54µm) followed by diploid (198.9±0.54µm) and triploid (258.4±0.54µm). Similar trend was noticed in case of plant growth, leaf quality and leaf yield parameters recorded indicating that triploid plants (T2) have shown an edge over diploid (T3), however, colchiploid mulberry plants (T1) have shown lower yield.