The present investigation was done to assess the genetic diversity of genotypes of G.hirsutum based on the agro-morphological characters. The experimental material for the present investigation comprised of 40 elite genotypes of upland cotton with one check (H 1098i). All the genotypes and checks were sown in the experimental area of the Cotton Section, Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar. Total of 8 agro-morphological characters were recorded the study viz., seed cotton yield/plant, lint yield/ plant, ginning % boll weight, seed index (g), lint index (g), plant height (cm) and number of bolls/plant. Analysis of variance revealed significant differences for all traits studied among the progenies. Value of GCV, PCV, heritability and genetic advance were higher to moderate for all the traits thus selection would be more effective for improvement for seed cotton yield per plant, lint yield per plant, lint index and number of bolls per plant. The genetic diversity analysis classified 41 genotypes into five clusters containing six to eleven genotypes. The cluster II was the largest (11) followed by cluster IV (9), both the clusters accounting for nearly 50% of the genotypes. The use of genotypes from cluster II, III and V would be useful to generate desirable genetic variability.