Twelve genotypes of cotton were evaluated at Viramgam, Dhandhuka and Kukada locations represent three different agroclimatic zone of Gujarat during kharif 2014-15. Analysis of variance indicated significant differences among the genotypes for seed cotton yield at individual locations except Kukada. It was found significant for pooled over the environments. Pooled analysis over three locations indicated that seven genotypes showed higher seed cotton yield performance than grand mean. The genotype GVhv 710 (2519 kg/ha) numerically produced highest seed cotton yield followed by best check ADC 1 (2507 kg/ha). Genotype GVhv 718 (1116 kg/ha) had recorded highest lint yield followed by entries GVhv 710 (1069 kg/ha) and GVhv 667 (1067 kg/ha). Highest span length of 25.7 mm was recorded by GVhv 796 but poor seed cotton yielder (1240 kg/ha). The genotype x environment interaction was found to be non-significant for seed cotton yield. Genotypes GVhv 710, GVhv 714, GVhv 717 and GVhv 718 were found stable with higher mean yield, regression coefficient and non-significant deviation from regression. These genotypes can be exploited for higher seed cotton yield under wider environmental conditions of rainfed area of Gujarat.