The research work was taken up to study the morphology and biochemical markers associated with the powdery mildew resistance in mungbean. Mungbean (Vigna radiata) is an important legume species belong to the family Fabaceae. One of the significantly important diseases of mungbean is powdery mildew caused by Erysiphe polygoni. The genotypes used for this study are SML 1082, TM-96-2, TARM-1, TARM-2 and TARM -18. Pathogen was taken from the powdery mildew infected field in the Department of Pulses, TNAU, Coimbatore. Morphology characters of greengram revealed that trichome density and trichome length, leaf cuticle thickness and epidermal layer thickness were negatively correlated with disease incidence. Biochemical parameters were studied before and after infection at three different periods of its growth (30, 45 and 60 Days after sowing). In biochemical parameters, phenol, protein and non-reducing sugar content present in the leaf were increased after infection compared to healthy but reducing sugar and protein content were decreased after infection. The sugar content was more in susceptible genotype than the resistant genotype whereas phenol and protein is more in resistant genotype. Among the five genotype, TARM-18 and followed by TM-96-2 shows higher attributes towards both morphological and biochemical markers against powdery mildew.