Biochemical studies were conducted in tender, medium and mature leaves in seven mulberry varieties viz., RFS175, MR2, S13, S30, S34, DD and M5 for eight biochemical parameters viz., moisture, protein, carbohydrate, crude fat, crude fibre, chlorophyll a and chlorophyll b under two management systems under rainfed condition. In this study the varieties and management were evaluated separately by conducting the experiment in split plot design. The study conducted from 2007-2010 at G.K.VK, Bangalore in red sandy clay loam soil under rainfed condition. In situ trenching, mulching and sub soil biomassing (M2 improved) practise in inter rows of mulberry garden showed significantly increased in the nutritive values compared to existing practice (M1, normal) viz., moisture percentage (M1: 69.85,66.83,62.97 & M2: 72.20,70.36,68.27) , protein percentage (M1 :18.57,15.70,15.59 & M2: 21.93,19.92,18.73), carbohydrate percentage ( M1:12.23,13.06, 14.09 & M2:14.82, 16.07,16.88), chlorophyll a (M1:1.59,1.71,1.87 and M2:1.68,1.75 and 1.98), chlorophyll b (0.42,0.44,0.47 and 0.44, 0.45 and 0.48), total mineral percentage (M1 11.00,12.90, 14.86,& M2 12.30,15.38,17.73) and crude fat percentage (M1 3.513, 3.804, 3.025 & 3.761, 4.011, 4.166) in tender, medium and mature leaves. Among the seven varieties evaluated the variety DD recorded significantly higher values for moisture, crude fat, total minerals and chlorophyll a. Whereas, Carbohydrate content was significantly higher in S13 compared to other varieties. Chlorophyll b was significantly higher in RFS175. The variety S34 recorded higher crude fibre. The moisture and protein showed a decreasing trend from tender to mature leaves where as other quality parameters showed a reverse trend. Compared to normal method M1 of cultivation in M2 method there is an increase in the biochemical constituents of leaf.