An incubation experiment was conducted to study the nitrification rate of Jatropha curcas cake and compared with Pongamia pinnata and Madhuca indica cakes. Estimation of ammonical, nitrate nitrogen were done at the intervals of 25, 50 and 75 days of incubation. Maximum ammonia formation during the first 25 days was reported in Jatropha seed cake (15.37 mg/g), higher over control (5.3 mg/100g) and minimum in Mahua seed cake (5.56). The control exhibited a gradual fall in ammonia formation i.e. 5.39 mg/100g to 1.97 mg/100g from 25 days to 75 days interval. The fall in the ammonia formation by the inorganic fertiliser (NH4)2SO4 was 13.48 mg/100g to 1.45 mg/100g at intervals of 25 to 75 days. Maximum production of nitrate nitrogen throughout the test period was recorded in treatment with inorganic fertilizer 18.24,19.98 and 23.84 mg/100g soil. Jatropha treatments i.e. Jatropha cake and methanol extracted cake resulted in 5.63 to 10.49 mg/100g nitrate nitrogen production. The minimum production was observed in control (2.28 5.01 and 6.22 mg/100g).