Vol. 12 (1&2 ) : Jan-April, 2021 Issue
Green Farming Vol. 12 (1&2 ) : 1-6; Jan.-April, 2021
Ovicidal properties of floral head, leaves and their tissue culture of Tagetes erecta, against Tribolium castaneum
VINAY KUMAR MEHRAa1*, KAVITA MATHURb2, T. N. NAGb3
aAnimal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal – 132001 (Haryana) bDept. of Biotechnology, M.N Institute of Applied Sciences, Maharaja Ganga Singh University, Bikaner-334001 (Raj.)
Designation : PhD Scholar *(vinay28mehra@yahoo.com), 2 Assistant Professor, 3 Director
Subject : Agriculture Botany
Paper No. : 21
Total Pages : 3
Received :
Revised accepted :
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Citation :
Mehra V.K., Mathur Kavita and Nag T.N. 2021. Ovicidal properties of floral head, leaves and their tissue culture of Tagetes erecta, against Tribolium castaneum Green Farming. Vol. 12 (1&2): 0000, Jan.-Feb. & March-April 2021.
ABSTRACT
Ovicidal properties of these insecticides such as pyrethrins have been detected in several plant species of Astaraceae family. Floral head and leaves of Tagetus erecta are collected and callus tissue of Tagetes erecta were raised and maintained on M.S medium supplemented with 2mg/l IAA, 2, 4-D & Kinetin. Six week old tissues maintained on MS medium were analyzed for their ovicidal properties against Tribolium castaneum. Adult of T.castaneum were collected from cereals, identified and cultured on rice. Aqueous extract were prepared from floral head and leaves and their cultures. The numbers of unhatched (dead) eggs were counted after the incubation period and the mortality percentage was calculated and corrected using Abbott's Formula. It was observed that extract of petals containing absolute concentration was highly toxic to the eggs recording a mortality of 82±1.14%. Mortality percentage decreased with decreasing concentration and the lowest mortality of 54±1.58% was observed at 25% concentration of leaves callus extract .It was also observed from the present findings that floral heads of T. erecta found to be better ovicidal properties when compared with their callus culture. It can be conclude that floral head and tissue culture of Astaraceae family have ovicidal properties against insects.
Key words :
Biopesticide, Insecticides, Ovicidal, Tagetus, Tissue culture, Tribolium castaneum