Vol. 6 (3) : May-June 2015 issue
Green Farming Vol. 6 (3) : 604-606 ; May-June, 2015
PCR detection of endosymbionts in whitefly collected from cassava and cassava based crops
JITENDRA KUMARa1*, R. RABINDRANa2 and PREM KUMARb3
aDeptt.of Plant Pathology, Center for Plant Protection Studies, Tamil Nadu Agri. Uni., Coimbatore - 641 003 (T. N.)
bDeptt. of Genetics and Plant Breeding, Institute of Agric. Sciences, Banaras Hindu Uni., Varanasi - 221 005 (U. P.)
Designation : 1,3Ph.D. Scholar *(jitpatho@gmail.com), 2Professor & Registrar
Subject : Agronomy and Crop Production
Paper No. : P-2309
Total Pages : 3
Received : 16 July 2014
Revised accepted : 14 March 2015
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Citation :
JITENDRA KUMAR, R. RABINDRAN and PREM KUMAR. 2015. PCR detection of endosymbionts in whitefly collected from cassava and cassava based crops. Green Farming Vol. 6 (3) : 604-606 ; May-June, 2015
ABSTRACT
The whitefly harbors many secondary endosymbionts viz., Rickettsia, Wolbachia and Arsenophonus which act as supplement to nutritionally imbalanced diet to the insect. In an attempt to study the occurrence of these endosymbionts in the whitefly, sample naturally occurring on cassava and other crop of cassava cropping system were collected from Coimbatore district and analyzed with the specific primers. These primers targeting the specific genes viz., 16S rRNA (Rickettsia), 16S rDNA (Wolbachia) and 16S-23S rDNA (Arsenophonus). These endosymbionts tested in the whiteflies, Rickettsia was present in whiteflies that were collected from tomato while Wolbachia and Arsenophonus were present in whiteflies collected from cassava and sunflower. On the other hand only Wolbachia was recorded on the cotton whitefly population. This preliminary study indicates the possibility of variation prevailing among the whiteflies populations of various crops in Tamil Nadu.
Key words :
Arsenophonus, Bemisia tabaci, Endosymbionts, PCR, Rickettsia, Whitefly, Wolbachia.
