Nigella sativa is an herbaceous and annual spice of the Rannunculaceae family. Investigation was undertaken to study the physical and nutrient composition including fatty acid and volatile oil profile of Nigella sativa seed spice from samples procured from NRCSS (National Research Center on Seed Spices, Ajmer) and local market. The results of the study indicated that this Nigella sativa spice contained substantial amounts of macro nutrients i.e., 16.31-16.52 per cent protein, 34-37 per cent fat, 6-7 per cent crude fiber and 4.15-4.18 per cent ash. It was also found to be abundant in several micronutrients. It was also found to be rich in unsaturated fatty acid such as linoleic acid to the extent of 79.20 per cent and oleic acid 10.8 per cent. Thymoquinone a major functional volatile oil fraction was found to be 37.70 percent. Results of the study uncover the secret of Nigella sativa as a bountiful source for many crucial health promoting nutrients. In addition, a high per cent of thymoquinone in the studied seed spice has several properties in disease management.