Three CMS lines i.e., Pant CMS 2A, Pusa 6A and UPRI 95- 17A and twenty two pollen parents of diverse origins were test crossed in order to identify potential restorers and maintainers. Spikelet fertility from 55 successful test crosses was able to differentiate parental lines into restorers, partial restorers, maintainers and partial maintainers. Spikelet fertility in the test cross progenies ranged from 7.00% to 91.87%. Understanding of genetic diversity among the parental lines is useful in rice breeding for effective selection of parental lines to maximize heterosis. Sixteen SSR primers were used to assess the extent of genetic diversity among thirty lines comprising three Cytoplasmic male sterile (CMS) lines (Pant CMS 2A, Pusa 6A and UPRI 95-17A), their corresponding maintainer lines (Pant CMS 2B, Pusa 6B and UPRI 95-17B) and twenty two test cross comprising 17 restorers, 33 partial restorers, one partial maintainers, four maintainers and one additional male sterile line Pant CMS 3A and corresponding maintainer line Pant CMS 3B. Total polymorphism ranged from 27.27 (RM 336) to 100 (RM276, RM327, RM287) with an average of 76.29. Primers RM 276, RM327 and RM287 found to be 100 % polymorphic. A total of 156 alleles were detected across the thirty rice genotypes with a mean of 9.75.lines. The PIC for all 16 primers varied from 0.384 (RM 592) to 0.919 (RM 499), with a mean of 0.754. Dendrogram revealed that minimum genetic similarity (0.75) was observed in UPR-3770-7-2-1 with UPR 3811-3-1-1, UPR 3876-9-1-1 with Pusa 6A and Pusa 6B, VNIIR 10184 (ANAIT) and Pant Dhan-18 in combination with Pant CMS 2B. UPR 3876-9-1-1 also observed 0.76 similaritiy with Pant CMS 2A and 0.75 with Pant CMS 2B. So these combinations can be used as heterotic parents in breeding improvement program of rice.