Microalgae (Chlorella sp.) was used for the production of biodiesel. Microalgae were harvested for 15 days interval up to 160 days. The productivity of algae was obtained as 1.5 g/liter/day. Physico-chemical properties of algae viz., bulk density, colour, particle size and physical state at room temperature as physical properties and moisture content, lipid content, protein content and carbohydrate as chemical properties of algae were determined and found to be 0.4 g/cc, green, 2-10 µm, dry powder form , 3.48 %, 8- 10 %, 51- 58 % and 12-17 % respectively. For oil extraction, two extraction solvent systems were used in which Hexane/ether (1:1, v/v) resulted in low lipid recovery i.e. 4.8 % (dry basis) than that of chloroform/methanol (2: 1, v/v) resulted 9.0% (dry basis). To develop process for biodiesel production from algae, three different transesterification processes were employed with 1:5 methanol to bio-oil ratio based on three different catalysts viz., alkali catalyst, acid catalyst and enzymatic catalyst with two different temperature i.e. 50°C and 60 °C. Alkali catalyst was resulted the highest yield of biodiesel to 92 % at 60 °C temperature.