The findings of the conducted experiment as a part of Long-term fertilizer experiment in Vertisol under soybean-wheat intensive cropping system during 2010-11 revealed that the increasing fertilizer levels significantly enhanced the crop yields, total S uptake and different S fractions (heat soluble, organic and total) even after repeated application of fertilizers and manure for the last 39 years. The available S and its fractions were found maximum in 100% NPK+FYM treated plot and minimum in control. The successive addition of sulphur fertilizer increased the available S status and S fractions from its initial level with the highest deposition (43.35 kg ha-1) in the treatment receiving 100% NPK+FYM followed by 150% NPK (38.67kg ha-1) addition. While the lowest status was recorded where S free fertilizers were continuously added 17.94 kg S ha-1 at the surface soil (0-20 cm). The value decreased significantly with increasing soil depth. The relationship between inorganic forms of S and availability of S showed that S application increased available S in the soil. Results also showed that, S fractions were significantly correlated with productivity of soybean and amongst the various forms of S, Available-S, Organic-S, Heat soluble-S and total-S was positively and significantly correlated with organic carbon. Exclusion of sulphur in the fertilizer schedule had deleterious effect on crop productivity as well as nutrient availability. The highest crop productivity of soybean and wheat 2.67 and 5.80 t ha-1 was recorded with balanced 100% NPK applied with FYM and the lowest 1.20 and 1.35 t ha-1 of soybean and wheat, respectively in control plot. These findings clearly indicated that balanced fertilizer use in conjunction with organic manure resulted in a sustainable productivity of crops.