Vol. 11 (6) : Nov - Dec 2020 Issue
Green Farming Vol. 11 (6 ) : 530-534 ; Nov-Dec, 2020
Profiling of flavonoids in Annona spp. fruit extracts using HPLC-MS/MS
MANJUNATH J SHETTYA1*, VASUDEVA K. RA2, SAKTHIVEL TB3, SURESH G. JA4, KRISHNA H. CC5, VISHNUVARDHANAD6 and ANILKUMAR SE7
a Dept. of Postharvest Technology, College of Horticulture, Bengaluru, 560065 b Division of Fruit Crops, Indian Institute Horticultural Research, Bengaluru, 560089 c Dept. of Postharvest Technology, College of Horticulture, Kolar, 563101 dRegional Horticulture Experimental Research Station (RHREC), Bengaluru, 560065 eKrishi Vigyan Kendra, Kolar, 563101 (Karnataka)
Designation : 1 *Ph.D. Scholar (*jshettymanju@gmail.com), 2 Professor, 3 Principal Scientist, 4 Asstt. Professor, 5 Assoc. Professor, 6 ADRE, 7 Scientist
Subject : Agriculture Botany
Paper No. :
Total Pages : 5
Received : 12 Nov. 2020
Revised accepted : 15 Dec. 2020
Get Access
Citation :
Shetty Manjunath J, Vasudeva K. R., Sakthivel T, Suresh G. J, Krishna H. C, Vishnuvardhana and Anilkumar S. 2020. Profiling of flavonoids in Annona spp. fruit extracts
using HPLC-MS/MS Green Farming Vol. 11 (6): 530-534. Nov. -Dec. 2020.
ABSTRACT
Total fifteen individual flavonoids were identified and quantified from the methanolic extracts of fruit pulp of Annona spp. by HPLC analysis. Chromatographic separation was performed on a C18 column (2.1 × 50 mm, 1.7 µm) with mobile phase consisting of 0.1% formic acid and 100% methanol at a flow rate of 0.3 mL/min. The analysis was performed using a triple quadrupole mass spectrophotometer (TQD-MS). The results indicated that flavonoids viz., catechin, epicatechin, myricetin, quercetin and luteolin were majorly found in annona fruits while epigallocatechin, rutin, naringenin, hesperidin, umbelliferone and kaempferol were insignificant. Therefore, annona fruits can be a potential candidate for further work to discover antioxidant and cytotoxic drugs from natural sources.
Key words :
Annona spp., Catechin, Epicatechin, Flavonoids, HPLC-MS/MS
