Vol. 9 (5) : September-October 2018 issue
Green Farming Vol. 9 (5) : 792-799 ; September-October, 2018
Prospecting male gametophytic selection for fusarium wilt resistance in carnation (Dianthus caryophyllus L.)
PURNACHANDRA GOWDA G.a1*, DHANANJAYA M.V.b2, DEVAPPA V.c3, FAKRUDIN B.d4, ASHWATH C.e5, SRIRAM S.f6 and RAJIV KUMARe7
aDept. of Floriculture & Landscape Architecture, cDeptt. of Plant Pathology, dDeptt. of Biotech. & Crop Improvement College of Horticulture, University of Horticultural Sciences, GKVK Post, Bengaluru - 560 065 (Karnataka),
bDivision of Ornamental Crops, eDivision of Floriculture and Medicinal Crops, fDivision of Plant Pathology, ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore - 560 089 (Karnataka)
Designation : 1Ph.D. Scholar *(purnachandra.gowda@gmail.com), 2,5,6,7Principal Scientist, 3,4Professor
Subject : Agriculture Botany
Paper No. : P-7408
Total Pages : 8
Received : 21 August 2018
Revised accepted : 04 September 2018
Get Access
Citation :
PURNACHANDRA GOWDA G., DHANANJAYA M.V., DEVAPPA V., FAKRUDIN B., ASHWATH C., SRIRAM S. and RAJIV KUMAR. 2018. Prospecting male gametophytic selection for fusarium wilt resistance in carnation (Dianthus caryophyllus L.). Green Farming Vol. 9 (5) : 792-799 ; September-October, 2018
ABSTRACT
An investigation was conducted on “Gametophytic selection for fusarium wilt resistance in carnation (Dianthus caryophyllus L.)”at the Division of Floriculture and Medicinal Crops, Indian Institute of Horticultural Sciences, Hessarghatta, Bangalore. Pollens of eight carnation genotypes were studied for their resistance against Fusarium wilt using mycotoxin; Fusaric acid in pollen germination media. The genotypes viz., IIHRCG- 201, IIHRCG- 202, IIHRCG- 205, IIHRCG- 206, IIHRCG- 207, IIHRCG- 208, IIHRCG- 209 and IIHRCG- 210 were assessed for their pollen germination and pollen tube growth in presence of Fusaric acid at different concentrations (0, 0.5, 1, 2, 5, 10, 25 and 50 %). Among the genotypes assessed, IIHRCG-202 recorded the highest germination per cent (33.15 %), whereas the lowest was observed in IIHRCG-209 (18.81 %). The pollen tube growth was recorded to be highest the same genotype IIHRCG-202 (152.81 µm), whereas the least tube growth was recorded in IIHRCG-201 (82.81 µm) respectively. The ability of the genotypes to resist the stress induced by fusaric acid was evaluated on the parameters; relative pollen germination and pollen tube growth over the control. The genotype IIHR-CG-202 recorded the lowest relative change in pollen germination per cent (68.44 %), which was on par with that of the genotypes IIHR-CG-207 (67.89 %) and IIHR-CG-208 (65.19 %) indicating significant level of resistance against Fusarium wilt.
Key words :
Carnation, Fusarium wilt, Fusaric acid, Genotypic resistance, Pollen germination.
