The field experiment to study the effect of organic manures and soil moisture regimes on nutrient availability and yield of mustard irrigated with different saline water was conducted to workout the effect on physico-chemical properties, nutrient availability, dynamics of soil microbial biomass and enzymes activities, yield, nutrient concentration and quality of mustard in loamy sand soil at Agronomy Farm, S.K.N. College of Agriculture, Jobner during rabi seasons of 2010-11 and 2011-12. The experiment comprising 27 treatment combination replicated three times, was laid out in split plot design with three levels of organic manures (control, FYM @ 10 t ha-1 and vermicompost @ 5 t ha-1), three levels of moisture regims (0.4, 0.6 and 0.8 IW/CPE) and three levels of saline water (control, 6, and 12 dSm -1) The highest N, P, K concentration, N, P and K uptake was recorded under vermicompost applied @ 5 t ha-1 over rest of the treatments. The highest N, P, K concentration, N, P and K uptake was recorded under 0.8 moisture regimes over rest of 0.4 and 0.6 moisture regimes. nutrient concentration viz., P and K, and uptake of N, P and K decreased significantly, while N concentration increased significantly with increasing level of saline water.