Water stress is a big problem that can occur periodically in all climatic zones, but mostly occurs with greater frequency in tropical and subtropical regions, causing physiological and biochemical damage to plants in ecosystems and agro-ecosystems. Silicon is identified as to ameliorate the harmful effects of various stresses on plants. Keeping the above facts in mind a pot experiment was conducted to study the effect of silixol (liquid source of silicon) under water stress in rice crop during kharif season of 2014 and 2015 at G.B. Pant University of Agriculture and Technology, Pantnagar. Water stress tolerant biochemical attributes like malondialdehyde content (MDA), proline content and superoxide dismutase (SOD) activity were analyzed during this experiment. Root growth is a physiological marker for the avoidance of water stress. It was observed that proline content, SOD activity and root length increased in silicon solubilizer (silixol) treated plants as compared to untreated rice genotypes. MDA is a product of lipid peroxidation and highly produced during stressed condition but with the application of silixol, its concentration was not high. Among all the genotypes, the minimum amount of MDA content was observed in rice genotype PA6444. Proline and SOD activity were observed maximum in rice genotype PA-6444. Maximum plant root length was recorded in rice genotype PA-6444. It was found that the rice genotype PA-6444 showed drought stress tolerant character with the application of silicon solubilizer. Rice genotype BPT-5204 recorded maximum MDA concentration and lowest proline and SOD activities. These results suggested that the beneficial role of silicon solubilizer for rice crop under drought stress. Increased proline and antioxidant content in the stressed genotypes of rice through silicon solubilizer (silixol) application may be an adaptation to overcome the drought stress conditions and can be applied to farmer's field after further validation.