Vol. 6 (1) : January-February 2015 issue
Green Farming Vol. 6 (1) : 17-20 ; January-February, 2015
Saponin, amylase inhibitor and trypsin inhibitor of pigeon pea [Cajanus cajan (L.) Millsp.] hybrids as affected by different soaking treatments
REENA DEVIa1, SHILPA CHAWLAb2*, A.K. SAXENAc3 and CHARUL CHAUDHARYd4
aDeptt. of Biochemistry, cDeptt. of Plant Breeding & Genetics, Punjab Agricultural University, Ludhiana - 140 004 (Punjab)
bDeptt. of Biochemistry, dCentre of Food Science & Techn., CCS Haryana Agricultural University, Hisar - 125 004 (Haryana)
Designation : 1,2,4Research Scholar *(sunsshine2009@gmail.com), 3Sr. Analyst
Subject : Plant Physiology, Crop Physiology and Bio-Chemistry
Paper No. : P-2100
Total Pages : 4
Received : 02 June 2014
Revised accepted : 04 December 2014
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Citation :
REENA DEVI, SHILPA CHAWLA, A.K. SAXENA and CHARUL CHAUDHARY. 2015. Saponin, amylase inhibitor and trypsin inhibitor of pigeon pea [Cajanus cajan (L.) Millsp.] hybrids as affected by different soaking treatments. Green Farming Vol. 6 (1) : 17-20 ; January-February, 2015
ABSTRACT
The present investigations were undertaken to ascertain the effect of different soaking treatment on saponin, amylase inhibitor and trypsin inhibitor of pigeon pea hybrids. Hybrids had considerable amount of antinutrients viz., amylase inhibitor activity (mean 31.84 units/g), trypsin inhibitor activity (mean 8.13 mg/g) and saponin content (mean 1.78 mg/g). These antinutritional factors decrease bioavailability of proteins by interacting with proteins and forming insoluble complexes. Twenty four hybrids were evaluated for reduction in saponin, amylase inhibitor and trypsin inhibitor on different soaking treatments. Soaking in distilled water and different salt solutions viz., 1% NaHCO3, MSS (1.5% NaHCO3+0.5% Na2CO3+0.75% citric acid) led to a significant (P< 0.05) reduction in saponin, amylase inhibitor and trypsin inhibitor. Soaking in MSS was found to be the most effective amongst various soaking methods which resulted in 65.4%, 23.5% and 20.22% reduction in saponin, amylase inhibitor and trypsin inhibitor respectively.
Key words :
Antinutrients, Amylase inhibitor, Pigeonpea hybrids, Soaking hybrids, Salt solutions, Saponin, Trypsin inhibitor.
