A pot experiment was conducted on wheat crop (cv PBW 550 and PDW 233) at Punjab Agricultural University, Ludhiana during rabi 2011 in loamy sand soil having pH 7.36 and low DTPA-Cu (0.18 mg kg-1 soil). The experiment was laid in CRD design with objective to compare the efficiency of seed priming, soil fertilization and foliar fertilization. Seed priming @ 0.03 and 0.06% CuSO4 with and without foliar application (0.1% CuSO4 + lime at 30, 40 and 50 days growth) were compared with soil application (4.5 mg Cu kg-1 soil) , seed coating (3g CuSO4kg-1 seed) and foliar application (0.1% CuSO4 + lime at 30, 40 and 50 days growth). Irrespective of the varieties, mean dry matter yield of 60 days old root & shoot and grain and straw yield at maturity increased significantly by 19.8, 25, 20.2 and 17.4% with soil application of Cu over control, respectively. The corresponding values observed for Cu seed priming with 0.03% CuSO4.5H2O + foliar application were 5.7, 16, 40.5 and 19.6%, respectively. The differences in the yield and Cu uptake by wheat with soil application and Cu seed priming with 0.03% CuSO4.5H2O with or without foliar application were not significant, thereby indicating that both these treatments were equally effective for Cu fertilization of wheat. Furthermore, the yields and Cu uptake by PDW 233 were significantly lower than that of PBW 550. These results indicated that either the Cu requirements of durum wheat may be lower than that of bread wheat or the translocation of Cu from root to shoot of PDW 233 may be affected due to lower production of root biomass. In the absence of any visual symptoms of Cu deficiency, an increased activity of ascorbate peroxidase in the leaves as a result of Cu fertilization suggested that wheat might have suffered from hidden hunger of Cu under the conditions where these investigations were undertaken.