Vol. 1 (4) : July-August 2010 issue
Green Farming Vol. 1 (4) : 336-340 (July-August, 2010) (New Series)
Studies on genetic variability in cowpea (Vigna unguiculata) through in vitro culture
K.D. DADMAL1*, U.R. AGARKAR2, N.S. NIKAS3 and G.K. PIWLATKAR4
Vivekanand Agriculture College, Hiwara (Bk.), Tq.- Mehkar Dist- Buldhana - 443 301 (Maharashtra)
Designation : 1Asstt. Professor (Agric. Botany, 2Asstt. Professor (Horti.),
3Asstt. Professor (Soil Sci. & Agric. Chemistry), 4Asstt. Professor (Agril. Engineering)
Subject : Crop Genetics and Plant Breeding
Paper No. :
Total Pages : 5
Received :
Revised accepted :
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Citation :
K.D. DADMAL, U.R. AGARKAR, N.S. NIKAS and G.K. PIWLATKAR. 2010. Studies on genetic variability in cowpea (Vigna unguiculata) through in vitro culture. Green Farming Vol. 1 (4) : 336-340 ; July-August, 2010 (New Series)
ABSTRACT
Pulse crops are the most versatile legumes with diversified uses as food, fodder, vegetable and green manuring crop. The hypocotyl explant of Konkan Sadabahar with 3.0 mg/l 2,4-D concentration and embryo explant of PCP-9757 with 2.0 mg/l 2,4-D concentration showed higher response for callus induction in MS medium. The maximum (9.00) shoots were regenerated in 5.0 mg/l BAP concentration by genotype BDN-1 using hypocotyl explant where as In MS medium the hypocotyl explant of BDN-1 and embryo explant of Konkan Safed and PCP-9719 regenerated maximum roots with 1.0 mg/l IBA.
Key words :
Cotyledon, Cowpea, Cultures, Explant, Hypocotyl, Shoot tip, Vigna unguiculata.
