The broadness application of organophosphorus compounds has abounded the number of its polluted areas. Bioremediation has widely focused on in situ bacterial degradation of organophosphorus residues in the world. Therefore, in this research six samples from different soil places randomly were for isolation using dimethoate pesticide containing mineral solution without supplementation. Eight isolated strains were selected according to their simultaneous optimal growth on mineral medium with dimethoate. The resistant strains were identified by two methods, the colony characteristics and biochemical convention. The identified strains belonged to Pseudomonas and Actinomycetes species. The maximum tolerant concentrations of dimethoate organophosphorus pesticides by these resistant strains were 100,300,500,700, and 900ppm. The resistance to the pesticides due to organ phosphorous degrading plasmids had the ability to express hydrolytic enzymes. Thus, certain environmental bacteria could be used as protection tools against bioremediation agents.